Journal: PLoS ONE
Article Title: Modulating the Adhesion of Haematopoietic Stem Cells with Chemokines to Enhance Their Recruitment to the Ischaemically Injured Murine Kidney
doi: 10.1371/journal.pone.0066489
Figure Lengend Snippet: HPC-7 expressed the main KC and SDF-1α receptors, CXCR2 (A) and CXCR4 (B) respectively. Topically treating a healthy kidney with KC [200 ng/ml; C] or SDF-1α [200 ng/ml; D] for 4 hours led to significant HPC-7 recruitment compared to the PBS control. Blocking CXCR2 (E) and CXCR4 (F) on HPC-7 prior to administration also decreased HPC-7 adhesion in vivo within the IR injured kidney. For all figures: PBS controls = solid line; kidney pre-treatments/mAb treated HPC-7 = dashed line. Results are presented as mean ± SEM (n≥4). *p<0.05, **p<0.01.
Article Snippet: Flow cytometry studies were conducted using fluorescein isothiocyanate (FITC)-conjugated isotype control rat IgG2b, phycoerythrin (PE)-conjugated isotype control IgG2b, FITC-anti-CD18 (GAME-46; Santa Cruz), PE-anti-CD44 (IM7), FITC-anti-CD49d (MFR4.B), FITC-CD184 (CXCR4; 2B11; eBioscience, San Diego, California, USA) and Phycoerythrin (PE)-conjugated rat monoclonal anti-mouse CXCR2 (242216; R&D Systems, UK).
Techniques: Control, Blocking Assay, In Vivo